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pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-cYFP-STR1g_STR2p-nYFP-STR2g
(Plasmid #254407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254407 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCambia2300
  • Total vector size (bp) 23929
  • Vector type
    Plant Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    STR promoter-nYFP-STR_STR2 promoter-cYFP-STR2
  • Promoter STR promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    M. truncatula BCP1 promoter - NLS-2X-mCherry
  • Promoter MtBCP1 promoter

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-cYFP-STR1g_STR2p-nYFP-STR2g was a gift from Maria Harrison (Addgene plasmid # 254407 ; http://n2t.net/addgene:254407 ; RRID:Addgene_254407)

  • For your References section:

    Yeast two-hybrid-sequencing and bifluorescence complementation resources for assessing protein-protein interactions in arbuscular mycorrhizal roots: CKL2 as a case study. Ivanov S, Muller LM, Lefevre FM, Harrison MJ. New Phytol. 2026 Feb;249(4):1592-1604. doi: 10.1111/nph.70832. Epub 2025 Dec 23. 10.1111/nph.70832 PubMed 41431834
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