Yeast two-hybrid-sequencing and bifluorescence complementation resources for assessing protein-protein interactions in arbuscular mycorrhizal roots: CKL2 as a case study.

Ivanov S, Muller LM, Lefevre FM, Harrison MJ
New Phytol. 2026 Feb;249(4):1592-1604. doi: 10.1111/nph.70832. Epub 2025 Dec 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
254406	pCambia2300-Aar1-ccdB-NLS2xmCH	Destination vector for bifluorescence complementation. The plasmid carries NLS-mCherry expressed from the Medicago BCP1promoter.
254407	pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-cYFP-STR1g_STR2p-nYFP-STR2g	BiFC plasmid with cYFP-STR and nYFP-STR2 expressed from their native promoters and MtBCP1-NLS- 2X mCherry.
254408	pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-nYFP-STR1g_STR2p-cYFP-STR2g	BiFC plasmid with nYFP-STR and cYFP-STR2 expressed from their native promoters and MtBCP1-NLS- 2X mCherry.