pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-nYFP-STR1g_STR2p-cYFP-STR2g
(Plasmid
#254408)
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PurposeBiFC plasmid with nYFP-STR and cYFP-STR2 expressed from their native promoters and MtBCP1-NLS- 2X mCherry.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254408 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCambia2300
- Total vector size (bp) 23930
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameStr promoter-cYFP-STR-STR2 promoter-nYFP-STR2
- Promoter STR promoter
Cloning Information for Gene/Insert 1
- Cloning method Other
- 5′ sequencing primer CTTTGATTTATATCCCCGTAGTGG
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameMtBCP1 promoter-NLS-2XmCherry
- Promoter MtBCP1
Cloning Information for Gene/Insert 2
- Cloning method Other
- 5′ sequencing primer GAGAGAGGGAGATGTGTTTTTAAGG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-nYFP-STR1g_STR2p-cYFP-STR2g was a gift from Maria Harrison (Addgene plasmid # 254408 ; http://n2t.net/addgene:254408 ; RRID:Addgene_254408) -
For your References section:
Yeast two-hybrid-sequencing and bifluorescence complementation resources for assessing protein-protein interactions in arbuscular mycorrhizal roots: CKL2 as a case study. Ivanov S, Muller LM, Lefevre FM, Harrison MJ. New Phytol. 2026 Feb;249(4):1592-1604. doi: 10.1111/nph.70832. Epub 2025 Dec 23. 10.1111/nph.70832 PubMed 41431834