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AAV-Gfa2-VIVIT-EGFP
(Plasmid #254430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254430 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    AAV2 packaging
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VIVIT-Green Fluorescent Protein
  • Alt name
    VIVIT-EGFP
  • Species
    Synthetic
  • Promoter Gfa2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The VIVIT-EGFP fusion blocks the calcineurin-NFAT interaction, blocking that pathway.

Also referenced in:

Furman JL, Sama DM, Gant JC, Beckett TL, Murphy MP, Bachstetter AD, Van Eldik LJ, Norris CM. Targeting astrocytes ameliorates neurologic changes in a mouse model of Alzheimer's disease. J Neurosci. 2012 Nov 14;32(46):16129-40. doi: https://doi.org/10.1523/jneurosci.2323-12.2012. PMID: 23152597.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Gfa2-VIVIT-EGFP was a gift from Christopher Norris (Addgene plasmid # 254430 ; http://n2t.net/addgene:254430 ; RRID:Addgene_254430)

  • For your References section:

    Blockade of Astrocytic Calcineurin/NFAT Signaling Helps to Normalize Hippocampal Synaptic Function and Plasticity in a Rat Model of Traumatic Brain Injury. Furman JL, Sompol P, Kraner SD, Pleiss MM, Putman EJ, Dunkerson J, Mohmmad Abdul H, Roberts KN, Scheff SW, Norris CM. J Neurosci. 2016 Feb 3;36(5):1502-15. doi: 10.1523/JNEUROSCI.1930-15.2016. 10.1523/JNEUROSCI.1930-15.2016 PubMed 26843634
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