pPiggyBac_NTVE(PABP)-dVSVG-FLAG
(Plasmid
#254461)
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PurposeTransposon encoding doxycycline-inducible NTVE(PABP) with FLAG tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254461 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPiggyBac
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Modifications to backboneCAG_Tet-On3G_P2A_Puromycin-N-acetyltransferase
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNTVE(PABP)_P2A_dVSV-G-FLAG
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SpeciesSynthetic
- Promoter TRE3G
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GAACGTATAAGCTTTAGGCGTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.11.622832 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPiggyBac_NTVE(PABP)-dVSVG-FLAG was a gift from Gil Westmeyer (Addgene plasmid # 254461 ; http://n2t.net/addgene:254461 ; RRID:Addgene_254461) -
For your References section:
Non-destructive transcriptomics via vesicular export. Armbrust N, Grosshauser M, Geilenkeuser J, Stroppel L, Jozinovic M, Levermann H, Panne T, Wissmann J, Goelitz L, Schmidt S, Orschmann T, Rusha E, Steinmassl E, Widenmeyer F, Warsing N, Sabry M, Sultanbai A, Santl T, Geerlof A, Berezin O, Bodea SV, Moretti A, Schneider S, Theis F, Gagneur J, Truong DJ, Westmeyer GG. Nat Commun. 2026 Apr 25;17(1):3812. doi: 10.1038/s41467-026-72072-w. 10.1038/s41467-026-72072-w PubMed 42034633