Non-destructive transcriptomics via vesicular export.
Armbrust N, Grosshauser M, Geilenkeuser J, Stroppel L, Jozinovic M, Levermann H, Panne T, Wissmann J, Goelitz L, Schmidt S, Orschmann T, Rusha E, Steinmassl E, Widenmeyer F, Warsing N, Sabry M, Sultanbai A, Santl T, Geerlof A, Berezin O, Bodea SV, Moretti A, Schneider S, Theis F, Gagneur J, Truong DJ, Westmeyer GG
Nat Commun. 2026 Apr 25;17(1):3812. doi: 10.1038/s41467-026-72072-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254460 | pLenti_NTVE(PABP) | Lentiviral transfer vector encoding doxycycline-inducible NTVE(PABP). Tet-On 3G and puromycin N-acetyltransferase are expressed via the mouse Pgk1 promoter. |
| 254461 | pPiggyBac_NTVE(PABP)-dVSVG-FLAG | Transposon encoding doxycycline-inducible NTVE(PABP) with FLAG tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter. |
| 254462 | pPiggyBac_NTVE(PABP)-dVSVG-HA | Transposon encoding doxycycline-inducible NTVE(PABP) with HA tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter. |
| 254463 | pPiggyBac_NTVE(PABP) | Transposon encoding doxycycline-inducible NTVE(PABP). Co-expression of PiggyBac transposase required. Tet-On 3G and green fluorescent protein mGreenLantern are co-expressed via the CAG promoter. |
| 254464 | pAAV_TRE3G-NTVE(PABP) | AAV transfer vector encoding TRE3G-driven NTVE(PABP) with a surface FLAG epitope tag. Requires co-transduction with AAVs expressing rtTA (e.g., Tet-On 3G) or tTA for dox-dependent expression. |
| 254465 | pscAAV_hSyn-TetOn3G | Self-complementary AAV transfer vector with oversized payload comprising neuron-specific hSyn1 promoter-driven Tet-On 3G and mScarlet-I3. For co-transduction with pAAV_TRE3G-NTVE(PABP). |