Anti-SEMA3A [IPI-SEM3A.17]
(Antibody
#254646)
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PurposeAnti-SEMA3A (Human) chimeric recombinant antibody with fused human variable and rabbit constant domains; specific for human and mouse SEMA3A. Does not cross-react with other SEMAs.
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Depositing Lab
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Recommended Applications
Image 1 (of 3): Immunocytochemistry
A) Immunofluorescence (IF) of ExpiCHO cells transfected with V5-tagged human and mouse SE…
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Credit: Institute for Protein Innovation (IPI)
Image 2 (of 3): Immunocytochemistry
Each graph depicts the combined quantification of multiple images of the same transfected…
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Credit: Institute for Protein Innovation (IPI)
Image 3 (of 3): Flow Cytometry
Anti-SEMA3A [IPI-SEM3A.17] (Addgene #254646) recognizes human and mouse SEMA3A. Histogra…
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Credit: Institute for Protein Innovation (IPI)
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Recombinant Antibody | 254646-rAb | 100 µg of purified recombinant antibody | $262 * | ||
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Features
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Source SpeciesO. cuniculus (rabbit)
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IsotypeIgG
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Light Chain Typekappa
Production & Usage
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Recommended Secondary AntibodyAnti-rabbit IgG
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PurificationPurification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
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Storage BufferPBS, pH 7.4
Delivery
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Concentration0.9–1.1 mg/mL
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StorageStore at 4 °C for up to 1 month. For long-term storage, aliquot and store at -20 °C. Avoid multiple freeze/thaw cycles.
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ShipmentBlue ice
Resource Information
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Persistent Resource IdentifierRRID:AB_3740894
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
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Trademarks:
- Zeocin® is an InvivoGen trademark.
Target Antigen
| Protein | Semaphorin-3A |
|---|---|
| Antigen Description | Purified recombinant fragment of H. sapiens (human) SEMA3A, corresponding to aa: 21–686. |
| Antigen Amino Acid Sequence |
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| Species | H. sapiens (human) |
| Additional Species Reactivity | M. musculus (mouse) |
| Gene | SEMA3A |
| Alternative Names |
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| External References |
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Applications (6)
Flow Cytometry
Immunocytochemistry
Depositor Comments
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-SEMA3A [IPI-SEM3A.17] - from Institute for Protein Innovation (IPI) (Addgene antibody # 254646 ; http://n2t.net/addgene:254646 ; RRID:AB_3740894) -
For your References section:
IPI Antibody Collection
This antibody was developed by the Institute for Protein Innovation (IPI).
Learn about the IPI Collection
The heavy chain and light chain amino acid sequences are available by request after purchase. Please submit requests to [email protected]. For all other questions, please send an email to [email protected] for assistance.