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pColADuet-DddA-DddI-WT
(Plasmid #254736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254736 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pColADuet-1
  • Backbone manufacturer
    LifescienceMarket, Cat. #PVT0105
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Recommend growing plasmid in BL21(DE3) cells.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    DddA
  • Species
    Simiaoa sunii
  • Tag / Fusion Protein
    • N-6His tag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    DddI
  • Species
    Simiaoa sunii
  • Insert Size (bp)
    387

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the protocol document linked above, in the Resource Information section.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pColADuet-DddA-DddI-WT was a gift from Andrew Stergachis (Addgene plasmid # 254736)

  • For your References section:

    Mapping single-cell diploid chromatin fiber architectures using DAF-seq. Swanson EG, Mao Y, Mallory BJ, Vollger MR, Bohaczuk SC, Oliveira CB, Lyon DB, Ranchalis J, Parmalee NL, Cohen BA, Bennett JT, Stergachis AB. Nat Biotechnol. 2025 Dec 3. doi: 10.1038/s41587-025-02914-3. 10.1038/s41587-025-02914-3 PubMed 41339527
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