pAAV-neuro-seeERv1.1
(Plasmid #254753)

• Purpose: Cloning optimized bifunctional estrogen and CRE reporter similar to v1
• Depositing Lab: J Edward van Veen
• Depositing Organization: University of California, Los Angeles (UCLA)
  Located in the United States of America
• Publication: Cara et al Neuron. 2026 Aug 28:S0896-6273(26)00609-4. doi: 10.1016/j.neuron.2026.07.031.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 2868
• Total vector size (bp): 6863
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Estrogen Response Element
• Alt name: ERE
• Species: Synthetic
• Insert Size (bp): 190
• Tag / Fusion Protein:
  • d2EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a minimally modified version of pAAV-neuroSeeERv1.0 (Addgene #254751) that replaces a region of duplicated sequence in both polyA's. This change is not expected to change function but reduces likelihood of recombination during plasmid amplification.

Please visit https://doi.org/10.64898/2025.12.14.694224 for bioRxiv preprint.