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Tol2-LyzC-PHAKT-mCerulean3
(Plasmid #254786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254786 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Tol2-lyzc
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHAKT-mCerulean3
  • Species
    Synthetic
  • Promoter Lyzc

Cloning Information

  • Cloning method Other

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wendell Lim, PHAKT-mCerulean3 from Addgene #50837

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Infusion Cloning. Please visit https://doi.org/10.1101/2025.03.06.641746 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-LyzC-PHAKT-mCerulean3 was a gift from Qing Deng (Addgene plasmid # 254786 ; http://n2t.net/addgene:254786 ; RRID:Addgene_254786)

  • For your References section:

    Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis. Wang T, Kim DH, Ding C, Wang D, Zhang W, Silic M, Cheng X, Shao K, Ku T, Zheng C, Xie J, Xiao S, Jayant K, Yuan C, Chubykin AA, Staiger CJ, Zhang G, Deng Q. J Cell Biol. 2026 Jan 5;225(1):e202503037. doi: 10.1083/jcb.202503037. Epub 2025 Nov 19. 10.1083/jcb.202503037 PubMed 41259080
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