miniCMV-(AausFP1)2-mDeg
(Plasmid
#254844)
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Purposeplasmid encodes (AausFP1)2-mDeg fluorogenic protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name(AausFP1)2-mDeg
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Nucleotide: PX401319
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miniCMV-(AausFP1)2-mDeg was a gift from Jiahui (Chris) Wu (Addgene plasmid # 254844 ; http://n2t.net/addgene:254844 ; RRID:Addgene_254844) -
For your References section:
Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation. Pham TG, Ajayi O, He J, Sagarbarria I, Hardy JA, Wu J. Nat Methods. 2026 Jan;23(1):165-174. doi: 10.1038/s41592-025-02905-x. Epub 2025 Dec 17. 10.1038/s41592-025-02905-x PubMed 41407926