Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation.
Pham TG, Ajayi O, He J, Sagarbarria I, Hardy JA, Wu J
Nat Methods. 2026 Jan;23(1):165-174. doi: 10.1038/s41592-025-02905-x. Epub 2025 Dec 17.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254843 | CMV-CyTERM-mTagBFP2-24xMS2 | an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of MS2 RNA tag |
| 254844 | miniCMV-(AausFP1)2-mDeg | plasmid encodes (AausFP1)2-mDeg fluorogenic protein |
| 254845 | CMV-CyTERM-mTagBFP2-24xPP7 | an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of PP7 RNA tag |
| 254846 | miniCMV-(AausFP1)2-pDeg | plasmid encodes (AausFP1)2-pDeg fluorogenic protein |
| 254847 | miniCMV-Stargazin-tdTomato-mDeg | plasmid encodes plasma membrane Stargazin-tdTomato-mDeg fluorogenic protein |
| 254848 | miniCMV-Stargazin-tdTomato-pDeg | plasmid encodes plasma membrane Stargazin-tdTomato-pDeg fluorogenic protein |