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pTwist-Lenti-TRE-Arf6-mScarletI3-Puro
(Plasmid #254857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254857 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist-Lenti-TRE
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 7625
  • Total vector size (bp) 8879
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arf6-mScarletI3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1224
  • GenBank ID
    NM_001663
  • Entrez Gene
    ARF6
  • Promoter tight TRE promoter (Tet-inducible)
  • Tag / Fusion Protein
    • mScarletI3 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-Lenti-TRE-Arf6-mScarletI3-Puro was a gift from Sean R Collins (Addgene plasmid # 254857 ; http://n2t.net/addgene:254857 ; RRID:Addgene_254857)

  • For your References section:

    Parallel CRISPR screens reveal pathways controlling the cell surface levels of the attractant receptor FPR1. Akdogan E, Lundgren SM, Kamber RA, Bassik MC, Collins SR. Commun Biol. 2026 Mar 25. doi: 10.1038/s42003-026-09878-3. 10.1038/s42003-026-09878-3 PubMed 41882347
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