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pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry
(Plasmid #254862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254862 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTBL2367, Addgene 226675
  • Backbone size w/o insert (bp) 2754
  • Total vector size (bp) 9939
  • Vector type
    Mammalian Expression ; PiggyBac integration

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
  • Species
    H. sapiens (human), Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    429

Gene/Insert 2

  • Gene/Insert name
    pCAG-LacI-rb_glob_polyA
  • Species
    H. sapiens (human), G. gallus (chicken), O. cuniculus (rabbit), Synthetic; E. coli
  • Insert Size (bp)
    3566

Gene/Insert 3

  • Gene/Insert name
    pEF1alpha-mCherry-BGH_polyA
  • Species
    H. sapiens (human), B. taurus (bovine), Synthetic; Discosoma sp.
  • Insert Size (bp)
    2153

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry was a gift from Chang Liu (Addgene plasmid # 254862 ; http://n2t.net/addgene:254862 ; RRID:Addgene_254862)

  • For your References section:

    Open-ended molecular recording of sequential cellular events into DNA. Loveless TB, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC. Nat Chem Biol. 2025 Apr;21(4):512-521. doi: 10.1038/s41589-024-01764-5. Epub 2024 Nov 14. 10.1038/s41589-024-01764-5 PubMed 39543397
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