pAVA3936 [PRPF39 minigene: exon8-intron8 including poison exon-exon9]
(Plasmid
#254879)
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PurposeExpresses EFS-driven PRPF39 minigene (exon8-poison exon-containing intron8-exon9)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneEFS-BGHpolyA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRPF39 minigene (exon8-poison exon-containing intton8-exon9)
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SpeciesH. sapiens (human)
- Promoter EFS
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCGTATATAAGTGCAGTAGTCG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAVA3936 [PRPF39 minigene: exon8-intron8 including poison exon-exon9] was a gift from Andrei Alexandrov (Addgene plasmid # 254879 ; http://n2t.net/addgene:254879 ; RRID:Addgene_254879)