Andrei Alexandrov Lab: TRNAU1AP and PRPF39 Establish Integrated Control over Processing of Most Abundant Human Non-coding RNAs
Panah M, Che R, Mirani B, Chen X, Luo H, Alexandrov A
Nat Commun. 2026, in press
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254878 | pAVA3932 [TRNAU1AP minigene: exon3-intron3 including poison exon-exon4] | Expresses EFS-driven TRNAU1AP minigene (exon3--poison exon-containing intron3-exon4) |
| 254879 | pAVA3936 [PRPF39 minigene: exon8-intron8 including poison exon-exon9] | Expresses EFS-driven PRPF39 minigene (exon8-poison exon-containing intron8-exon9) |
| 254880 | pAVA4173 [CMV-TRNAU1AP(Δ184-278)-FLAG] | Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ184-278) |
| 254881 | pAVA4176 [CMV-TRNAU1AP(Δ248-278)-FLAG] | Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ248-278) |
| 254882 | pAVA3920 [CMV-6cMyc-PRPF39] | Expresses CMV-driven N-terminal 6cMyc-tagged PRPF39 |
| 254883 | LentiCRISPR-B_2xsgRNAs_TRNAU1AP (pAVA3291) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting TRNAU1AP |
| 254885 | LentiCRISPR-B_2xsgRNAs_PRPF39 (pAVA3245) | Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting PRPF39 |
| 254886 | LentiCRISPR-B_sgRNA_WDR61 (pAVA3321) | Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting WDR61 |
| 254887 | LentiCRISPR-B (pAVA2834) | Lentiviral vector expressing Cas9-P2A-Blasticidin without sgRNA used as backbone |