LentiCRISPR-B_2xsgRNAs_PRPF39 (pAVA3245)
(Plasmid
#254885)
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PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting PRPF39
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneLentiCRISPR-B
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Vector typeLentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTwo sgRNAs targeting PRPF39
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gRNA/shRNA sequenceGCTTCAATATGTAGAACAGG/gatgtacgcacCTCATCTGA
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SpeciesH. sapiens (human)
- Promoter U6/7SK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTGACGTAGAAAGTAATAATTTCTTGGGTAG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LentiCRISPR-B_2xsgRNAs_PRPF39 (pAVA3245) was a gift from Andrei Alexandrov (Addgene plasmid # 254885 ; http://n2t.net/addgene:254885 ; RRID:Addgene_254885)
