pEF6_pro_mIL1b-V5 (KS1047)
(Plasmid #254940)

• Purpose: Murine IL-1b precursor (pro-IL-1b) with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression.
• Depositing Lab: Kate Schroder
• Publication: Boucher et al J Exp Med. 2018 Mar 5;215(3):827-840. doi: 10.1084/jem.20172222. Epub 2018 Feb 6.

Backbone

• Vector backbone: pEF6
• Backbone size w/o insert (bp): 5839
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Il1b
• Species: M. musculus (mouse)
• Insert Size (bp): 858
• Entrez Gene: Il1b (a.k.a. IL-1beta, Il-1b)
• Promoter: EF-1a, T7
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 (TAATACGACTCACTATAGG)
• 3′ sequencing primer: BGH (TAGAAGGCACAGTCGAGG)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert has Kozak, V5 and STOP.