Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity.
Boucher D, Monteleone M, Coll RC, Chen KW, Ross CM, Teo JL, Gomez GA, Holley CL, Bierschenk D, Stacey KJ, Yap AS, Bezbradica JS, Schroder K
J Exp Med. 2018 Mar 5;215(3):827-840. doi: 10.1084/jem.20172222. Epub 2018 Feb 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254932 | pMIG (KS57) | pMSCV-derived retroviral expression vector with eGFP on IRES (empty vector) |
| 254933 | pMIG_mCasp1 (KS60) | Murine caspase-1 (Casp1) in pMIG, a pMSCV-derived retroviral expression vector with eGFP on IRES |
| 254934 | pMIG_mCasp1(C284A) (KS61) | Murine caspase-1 (Casp1) catalytic mutant (C284A) in pMIG, a pMSCV-derived retroviral expression vector with eGFP on IRES |
| 254936 | pEF6_DmrB-[dltCARD]mCasp1(E102D103TRB_E121D122AA) (KS655) | Murine Casp1 with its CARD replaced by DmrB, the 1st CDL cleavage site (E102D103) replaced by a Thrombin site, and the 2nd CDL cleavage site uncleavable (E121D122AA), in pEF6. |
| 254938 | pEF6_DmrB-[dltCARD]mCasp1 (KS1015) | Murine caspase-1 (Casp1) with its CARD domain replaced by the inducible homodimerization domain DmrB, in pEF6 for mammalian and bacterial expression. |
| 254939 | pEF6_DmrB-[dltCARD]mCasp1(C284A) (KS1017) | Catalytic mutant (C284A) of murine caspase-1 with its CARD domain replaced by the inducible homodimerization domain DmrB, in pEF6 for mammalian and bacterial expression. |
| 254940 | pEF6_pro_mIL1b-V5 (KS1047) | Murine IL-1b precursor (pro-IL-1b) with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. |
| 254941 | pEF6_pro_mIL18-V5 (KS1141) | Murine IL-18 precursor (pro-IL-18) with a C-terminal V5 tag in pEF6 for mammalian and bacterial expression. |
| 254943 | pGEX6p1_GST-HRV3C-DmrB-[dltCARD]mCasp1(E102D103TEV)-8xHis (KS585) | Murine caspase-1 with the E102/D103 autocleavage site replaced by a TEV site, CARD replaced by DmrB, N-terminal GST followed by a PreScission (HRV3C) site, and a C-terminal 8xHis tag. |