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AAVS1-CMV-V5-ZSWIM8
(Plasmid #254997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254997 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    AAVS1-CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-ZSWIM8
  • Species
    H. sapiens (human)
  • Mutation
    NA
  • Entrez Gene
    KIAA0913 (a.k.a. FLJ34302, MGC168869, MGC168870)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-CMV-V5-ZSWIM8 was a gift from Joshua Mendell (Addgene plasmid # 254997 ; http://n2t.net/addgene:254997 ; RRID:Addgene_254997)

  • For your References section:

    A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming. Han J, LaVigne CA, Jones BT, Zhang H, Gillett F, Mendell JT. Science. 2020 Dec 18;370(6523). pii: science.abc9546. doi: 10.1126/science.abc9546. Epub 2020 Nov 12. 10.1126/science.abc9546 PubMed 33184234
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