XLone-EPB41L4A-AS1 without SNORA13
(Plasmid
#255001)
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PurposeExpress EPB41L4A-AS1 with SNORA13 deleted in the intron in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 5607
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPB41L4A-AS1 without SNORA13
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SpeciesH. sapiens (human)
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Insert Size (bp)4160
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MutationSNORA13 deleted in the intron
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Entrez GeneEPB41L4A-AS1 (a.k.a. C5orf26, NCRNA00219, TIGA1)
- Promoter TRE3G
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 FWD
- 3′ sequencing primer M13 REV
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XLone-EPB41L4A-AS1 without SNORA13 was a gift from Joshua Mendell (Addgene plasmid # 255001 ; http://n2t.net/addgene:255001 ; RRID:Addgene_255001)