Joshua Mendell Lab: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence.
Cheng Y, Wang S, Zhang H, Lee JS, Ni C, Guo J, Chen E, Wang S, Acharya A, Chang TC, Buszczak M, Zhu H, Mendell JT
Cell. 2024 Aug 22;187(17):4770-4789.e23. doi: 10.1016/j.cell.2024.06.019. Epub 2024 Jul 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255000 | XLone-EPB41L4A-AS1 with SNORA13 | Express EPB41L4A-AS1 with SNORA13 located in the intron in mammalian cells |
| 255001 | XLone-EPB41L4A-AS1 without SNORA13 | Express EPB41L4A-AS1 with SNORA13 deleted in the intron in mammalian cells |
| 255002 | XLone-EPB41L4A-AS1 with SNORA13 Mut | Express EPB41L4A-AS1 with SNORA13 mutated at nucleotides 8-12 and 45-49 within the intron in mammalian cells |
| 255003 | PLKO.1-SNORA13-WT | Express SNORA13 in mammalian cells |
| 255004 | PLKO.1-SNORA13-H/ACA Mut | Express SNORA13 with mutations at the H box and ACA box in mammalian cells |
| 255005 | PLKO.1-SNORA13-H/ACA Del | Express SNORA13 with deletions at the H box and ACA box in mammalian cells |
| 255006 | pcDNA3-Renilla-FLAG-firefly | Used as a control for Plasmid pcDNA3 Renilla-FLAG-firefly-K529E |
| 255007 | pcDNA3-Renilla-FLAG-firefly-K529E | Used to test the rate of amino acid misincorporation in mammalian cells |