pcDNA3-Renilla-FLAG-firefly
(Plasmid
#255006)
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PurposeUsed as a control for Plasmid pcDNA3 Renilla-FLAG-firefly-K529E
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5350
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla-FLAG-firefly
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SpeciesSynthetic
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Insert Size (bp)2718
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFlag (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Renilla-FLAG-firefly was a gift from Joshua Mendell (Addgene plasmid # 255006 ; http://n2t.net/addgene:255006 ; RRID:Addgene_255006)
