PLKO.1-SNORA13-H/ACA Mut
(Plasmid
#255004)
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PurposeExpress SNORA13 with mutations at the H box and ACA box in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Texas Southwestern Medical Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePLKO.1
- Backbone size w/o insert (bp) 7036
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNORA13-H/ACA Mut
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SpeciesH. sapiens (human)
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Insert Size (bp)133
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MutationH box and ACA box
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Entrez GeneSNORA13 (a.k.a. ACA13)
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LKO.1 5'
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PLKO.1-SNORA13-H/ACA Mut was a gift from Joshua Mendell (Addgene plasmid # 255004 ; http://n2t.net/addgene:255004 ; RRID:Addgene_255004) -
For your References section:
A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Cheng Y, Wang S, Zhang H, Lee JS, Ni C, Guo J, Chen E, Wang S, Acharya A, Chang TC, Buszczak M, Zhu H, Mendell JT. Cell. 2024 Aug 22;187(17):4770-4789.e23. doi: 10.1016/j.cell.2024.06.019. Epub 2024 Jul 8. 10.1016/j.cell.2024.06.019 PubMed 38981482