PLKO.1-SNORA13-H/ACA Del
(Plasmid #255005)

• Purpose: Express SNORA13 with deletions at the H box and ACA box in mammalian cells
• Depositing Lab: Joshua Mendell
• Publication: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. (unpublished)

Backbone

• Vector backbone: PLKO.1
• Backbone size w/o insert (bp): 7036
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNORA13-H/ACA Del
• Species: H. sapiens (human)
• Insert Size (bp): 124
• Mutation: H box and ACA box
• Entrez Gene: SNORA13 (a.k.a. ACA13)
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: LKO.1 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry