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pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG
(Plasmid #255017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255017 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUra4AfeI
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P.cdc2(355-1,AfeImut)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1
  • Species
    S. pombe (fission yeast)
  • Mutation
    1 nucleotide removed in cdc2 promoter to abolish AfeI site
  • Promoter Spom\P.cdc2

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derived from pUra4AfeI, Addgene 133467, by Aleksandar Vjestica, Sophie Martin, et al., doi: 10.1242/jcs.240754 Please visit https://doi.org/10.64898/2026.03.09.710516 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG was a gift from Silke Hauf (Addgene plasmid # 255017 ; http://n2t.net/addgene:255017 ; RRID:Addgene_255017)

  • For your References section:

    Establishing MS2–MCP-based single-molecule RNA visualization in Schizosaccharomyces pombe. Douglas E. Weidemann, Sarah C. Turner, Samir G. Chethan, Silke Hauf. Journal of Cell Science 10.1242/jcs.264809
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