Establishing MS2-MCP-based single-molecule RNA visualization in Schizosaccharomyces pombe.
Weidemann DE, Turner SC, Chethan SG, Hauf S
J Cell Sci. 2026 Jul 1;139(13):jcs264809. doi: 10.1242/jcs.264809. Epub 2026 Jul 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255008 | pUC-BamHI-24xMS2V6-EcoRI | adding homology regions to 24xMS2V6 |
| 255009 | pUC-BamHI-12xMS2V6-EcoRI | adding homology regions to 12xMS2V6 |
| 255010 | pUra4-Plon1-MCP-NLS-tdSG | expression of MCP-NLS-tandemStayGold from weak P.lon1 promoter |
| 255011 | pUra4-Pmad3-MCP-NLS-tdSG | expression of MCP-NLS-tandemStayGold from weak P.mad3 promoter |
| 255012 | pUra4-Ppak1-MCP-NLS-tdSG | expression of MCP-NLS-tandemStayGold from weak P.pak1 (P.shk1) promoter |
| 255013 | pUra4-Pcdc2short-MCP-NLS-tdSG | expression of MCP-NLS-tandemStayGold from medium-strength P.cdc2 promoter |
| 255014 | pUra4-Pmad3-MCP-2xNLS-NES-tdSG | expression of MCP-2xNLS-NES-tandemStayGold from weak P.mad3 promoter |
| 255015 | pUra4-Pmad3-MCP-2xNLS-2xNES-tdSG | expression of MCP-2xNLS-2xNES-tandemStayGold from weak P.mad3 promoter |
| 255016 | pUra4-Pcdc2short-MCP-2xNLS-NES-tdSG | expression of MCP-2xNLS-NES-tandemStayGold from medium-strength P.cdc2 promoter |
| 255017 | pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG | expression of MCP-2xNLS-2xNES-tandemStayGold from medium-strength P.cdc2 promoter |
| 255018 | pUra4-Pmad3-stdMCP-NLS-tdSG | expression of tandemMCP-NLS-tandemStayGold from weak P.mad3 promoter |