pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP
(Plasmid
#255027)
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PurposeLentiviral plasmid designed to express a SaCas9 compatible gRNA expression cassette and dSaCas9-KRAB-T2A-GFP protein; Similar to Plasmid #162334, but with GFP instead of Puro
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLV
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedSaCas9
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SpeciesStaphylococcus aureus
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP was a gift from Jonathan Ploski (Addgene plasmid # 255027 ; http://n2t.net/addgene:255027 ; RRID:Addgene_255027)
