Jonathan Ploski Lab: Ploski Lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183704 | pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short) | AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit |
| 183705 | pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short) | AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit |
| 183707 | pLenti-0.5Syn-rtTA-WPRE-pA | Lenti vector designed to express rtTA from a 0.5 Synapsin promoter |
| 183708 | pAAV-CamkII-tTA-2A-E2-Crimson-WPRE-pA | AAV designed to express tTA and-E2 Crimson from a CamKIIa promoter |
| 183709 | pAAV-CMV-rtTA-IRES-GFP-WPRE-pA | AAV designed to express rtTA and eGFP from a CMV promoter |
| 255027 | pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP | Lentiviral plasmid designed to express a SaCas9 compatible gRNA expression cassette and dSaCas9-KRAB-T2A-GFP protein; Similar to Plasmid #162334, but with GFP instead of Puro |
| 255028 | pAAV-BiSSTe4-mELFN1-Myc-Venus-pA | AAV plasmid encoding mouse Elfn1 with a Myc Tag and Venus c-terminal fusion controlled from an SST-specific neuronal enhancer, BiSSTe4 |
| 255029 | pAAV-BiSSTe4-Venus-pA | AAV plasmid encoding Venus controlled from an SST-specific neuronal enhancer, BiSSTe4 |
| 255030 | pAAV-CMV-dSaCas9-KRAB(Zim3)-pA | AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled by a CMV promoter |
| 255031 | pAAV-BiSSTe4-dSaCa9-KRAB(Zim3)-pA | AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled from an SST-specific neuronal enhancer, BiSSTe4 |
| 255032 | pLenti_U6-DR30(SapI)_CMV-GFP-WPRE | Lentiviral plasmid designed to express an RfxCas13d compatible gRNA from a U6 promoter and GFP from a CMV promoter |
| 255033 | pLenti-CMV-CasRx-T2A-mCherry-WPRE | Lentiviral plasmid designed to express an RfxCas13d/CasRx-T2A-mCherry gene from a CMV promoter |
| 255034 | pLenti_EF1a-mCherry-P2A-hfCas13d-2xNLS-3xFLAG | Lentiviral plasmid designed to express an mCherry-P2A-high fidelity (hf) Cas13d; similar to plasmid #155305, but encoding hfCas13 instead of CasRx |