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pBK lpp MaPylT glnS AST
(Plasmid #255035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255035 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 3034
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M. alvus pyrrolysyl synthetase-AST
  • Insert Size (bp)
    828
  • Mutation
    L125A, N166S, V168T

Gene/Insert 2

  • Gene/Insert name
    tRNAMaPyl
  • Insert Size (bp)
    71

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBK lpp MaPylT glnS AST was a gift from Abhishek Chatterjee (Addgene plasmid # 255035 ; http://n2t.net/addgene:255035 ; RRID:Addgene_255035)

  • For your References section:

    A Translation-Independent Directed Evolution Strategy to Engineer Aminoacyl-tRNA Synthetases. Soni C, Prywes N, Hall M, Nair MA, Savage DF, Schepartz A, Chatterjee A. ACS Cent Sci. 2024 May 20;10(6):1211-1220. doi: 10.1021/acscentsci.3c01557. eCollection 2024 Jun 26. 10.1021/acscentsci.3c01557 PubMed 38947215
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