A Translation-Independent Directed Evolution Strategy to Engineer Aminoacyl-tRNA Synthetases.
Soni C, Prywes N, Hall M, Nair MA, Savage DF, Schepartz A, Chatterjee A
ACS Cent Sci. 2024 May 20;10(6):1211-1220. doi: 10.1021/acscentsci.3c01557. eCollection 2024 Jun 26.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253740 | pEvol sfGFP WT | WT reporter plasmid |
| 253742 | pBK lpp MaPylT glnS MaPylRS | Plasmid encoding M. alvus pyrrolysyl synthetase WT and tRNA |
| 253743 | pBK lpp MaPylT glnS LAC | Plasmid encoding mutant M alvus pyrrolysyl synthetase-LAC and tRNA for m-iodophenylalanine |
| 253744 | pBK lpp MaPylT glnS LSV | Plasmid encoding mutant M alvus pyrrolysyl synthetase-LSV and tRNA for m-iodophenylalanine |
| 253745 | pBK lpp MaPylT glnS ASV | Plasmid encoding mutant M alvus pyrrolysyl synthetase-ASV and tRNA for m-iodophenylalanine |
| 255035 | pBK lpp MaPylT glnS AST | Plasmid encoding mutant M alvus pyrrolysyl synthetase-AST and tRNA for o-nitrophenylalanine |
| 256311 | pBK lpp MaPylT metG ASV | Plasmid encoding mutant M alvus pyrrolysyl synthetase-ASV and tRNA for o-cyanophenylalanine |