K11_pET-28a(+)
(Plasmid
#255041)
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PurposeRecombinant tau construct for seeding activity assays and other applications.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemodified tau protein (K11)
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SpeciesH. sapiens (human)
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Entrez GeneMAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K11_pET-28a(+) was a gift from Byron Caughey (Addgene plasmid # 255041 ; http://n2t.net/addgene:255041 ; RRID:Addgene_255041) -
For your References section:
Classification of tauopathies from human brain homogenates through salt-modulated tau amplification. Santambrogio A, Metrick MA 2nd, Xu P, Gallagher NCT, Koga S, Ghetti B, Dickson DW, Caughey B, Vendruscolo M. Alzheimers Dement. 2026 Feb;22(2):e71112. doi: 10.1002/alz.71112. 10.1002/alz.71112 PubMed 41685551