K12/A322+His_pET-28a(+)
(Plasmid #255042)

• Purpose: Recombinant tau construct for seeding activity assays and other applications.
• Depositing Lab: Byron Caughey
• Publication: Santambrogio et al Alzheimers Dement. 2026 Feb;22(2):e71112. doi: 10.1002/alz.71112.

Backbone

• Vector backbone: pET-28a(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: modified tau protein (K12)
• Species: H. sapiens (human)
• Mutation: The K12 tau fragment (residues 244-275 and 306-400 of the full-length human tau sequence) with an alanine mutation at residue 322.
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A cloning cassette encoding the K12 tau fragment (residues 244-275 and 306-400 of the full-length human tau sequence) with an alanine mutation at residue 322, thereby rendering it cysteine-free (K12CF), was introduced between the Ndel and XhoI sites of the pET28 bacterial vector resulting in a histidine tag at the N-terminal (K12CFh). The cysteine was mutated in order to prevent disulfide-mediated dimerization of the K12 molecules.