YIplac211-SGM1(-Ypt6_BD)-FRBx2-HA
(Plasmid
#255060)
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PurposeRemove the Sgm1 Ypt6 binding domain sequence and insert a C-terminal FRBx2-HA tag at SGM1 by popin/popout.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneYIplac211
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSgm1(-Ypt6_BD)-FRBx2-HA
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SpeciesS. cerevisiae (budding yeast)
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MutationSgm1 Ypt6 binding domain removed
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YIplac211-SGM1(-Ypt6_BD)-FRBx2-HA was a gift from Benjamin Glick (Addgene plasmid # 255060 ; http://n2t.net/addgene:255060 ; RRID:Addgene_255060)