Benjamin Glick Lab: Glick Lab Plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
21770	YIPlac204TKC-DsRed-Express2-HDEL
25444	pHS12-mCherry
255054	pUC19-KanMX-msGFP2-YPT6	Integrate an N-terminally tagged msGFP2-Ypt6 sequence at the YPT6 locus. This is a second copy integration.
255055	YIplac211-HaloTag-IMH1	Insert an N-terminally tagged HaloTag-Imh1 sequence at the IMH1 locus. This is a second copy integration.
255056	YIplac211-HaloTag-SGM1	Insert an N-terminally tagged HaloTag-Sgm1 sequence at the SGM1 locus. This is a second copy integration.
255057	YIplac211-IMH1(-GRIP)-FRBx2-HA	Remove the Imh1 GRIP domain sequence and insert a C-terminal FRBx2-HA tag at IMH1 by popin/popout.
255058	YIplac211-IMH1(-GRIP)-msGFP2-FRBx2-HA	Remove the Imh1 GRIP domain sequence and insert a C-terminal msGFP2-FRBx2-HA tag at IMH1 by popin/popout.
255059	YIplac211-msGFP2-IMH1	Insert an N-terminally tagged msGFP2-Imh1 sequence at the IMH1 locus. This is a second copy integration.
255060	YIplac211-SGM1(-Ypt6_BD)-FRBx2-HA	Remove the Sgm1 Ypt6 binding domain sequence and insert a C-terminal FRBx2-HA tag at SGM1 by popin/popout.
255061	YIplac211-SGM1(-Ypt6_BD)-msGFP2-FRBx2-HA	Remove the Sgm1 Ypt6 binding domain sequence and insert a C-terminal msGFP2-FRBx2-HA tag at SGM1 by popin/popout.
255062	YIplac211-VPS10-FRBx2-HA-msGFPx3	Insert a C-terminal FRBx2-HA-msGFPx3 tag at VPS10 by popin/popout.
255063	YIplac211-VPS10-FRBx2-HA	Insert a C-terminal FRBx2-HA tag at VPS10 by popin/popout.
255064	YIplac211-VPS10-HaloTagx2	Insert a C-terminal HaloTagx2 at VPS10 by popin/popout.
255065	YIplac211-VPS52-FRBx2-HA	Insert a C-terminal FRBx2-HA tag at VPS52 by popin/popout.
255066	YIplac211-VPS52-HaloTagx2	Insert a C-terminal HaloTagx2 at VPS52 by popin/popout.
255067	YIplac211-VPS52-msGFP2x2	Insert a C-terminal msGFP2x2 tag at VPS52 by popin/popout.
255068	YIplac211-VPS54-msGFP2x3	Insert a C-terminal msGFP2x3 tag at VPS54 by popin/popout.