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Benjamin Glick Lab: Glick Lab Plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
21770YIPlac204TKC-DsRed-Express2-HDEL
25444pHS12-mCherry
255054pUC19-KanMX-msGFP2-YPT6Integrate an N-terminally tagged msGFP2-Ypt6 sequence at the YPT6 locus. This is a second copy integration.
255056YIplac211-HaloTag-SGM1Insert an N-terminally tagged HaloTag-Sgm1 sequence at the SGM1 locus. This is a second copy integration.
255057YIplac211-IMH1(-GRIP)-FRBx2-HARemove the Imh1 GRIP domain sequence and insert a C-terminal FRBx2-HA tag at IMH1 by popin/popout.
255058YIplac211-IMH1(-GRIP)-msGFP2-FRBx2-HARemove the Imh1 GRIP domain sequence and insert a C-terminal msGFP2-FRBx2-HA tag at IMH1 by popin/popout.
255059YIplac211-msGFP2-IMH1Insert an N-terminally tagged msGFP2-Imh1 sequence at the IMH1 locus. This is a second copy integration.
255060YIplac211-SGM1(-Ypt6_BD)-FRBx2-HARemove the Sgm1 Ypt6 binding domain sequence and insert a C-terminal FRBx2-HA tag at SGM1 by popin/popout.

Antibodies from Article