pKb-POC1
(Plasmid #255129)

• Purpose: Plant expression of rice codon optimized OpenCRISPR1 (OC1). Includes gRNA scaffold.
• Depositing Lab: Kutubuddin Molla
• Publication: Das et al New Phytol. 2026 May 21. doi: 10.1111/nph.71272.

Backbone

• Vector backbone: pRGEB32
• Backbone manufacturer: Yinong Yang
• Backbone size w/o insert (bp): 11763
• Total vector size (bp): 15900
• Modifications to backbone: SpCas9 was replaced by rice codon optimized OpenCRISPR1
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rice codon optimized pOC1
• Alt name: Rice codon optimized OpenCrispr-1
• Species: Synthetic
• Insert Size (bp): 4136
• Promoter: Rice ubiquitin promoter for pOC1 and OsU3 promoter for guide RNA expression

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstBI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry