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pKb-POC1-CBE
(Plasmid #255131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255131 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PE3max_pG3H
  • Backbone manufacturer
    Qijun Chen
  • Backbone size w/o insert (bp) 8942
  • Total vector size (bp) 14301
  • Modifications to backbone
    The prime editing cassette was replaced by A3A(Y130F)-OpenCRISPR1 D10A-UGI
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A(Y130F) fused at 5' of the Rice codon optimized pOC1 D10A and UGI at the 3' end
  • Alt name
    A3A(Y130F)-OpenCRISPR-1 D10A-UGI
  • Species
    Synthetic
  • Insert Size (bp)
    5359
  • Promoter Maize ubiquitin promoter for A3A(Y130F)-OpenCRISPR1 D10A-UGI and OsU3 promoter for guide RNA expression
  • Tag / Fusion Protein
    • NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKb-POC1-CBE was a gift from Kutubuddin Molla (Addgene plasmid # 255131 ; http://n2t.net/addgene:255131 ; RRID:Addgene_255131)

  • For your References section:

    AI-designed OpenCRISPR-1 performs robust knockout, base editing, and prime editing in rice. Das P, Saha R, Panda D, Ghosh C, Avinash SP, Panda S, Baig MJ, Molla KA. New Phytol. 2026 May 21. doi: 10.1111/nph.71272. 10.1111/nph.71272 PubMed 42169438
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