pMTK_026-Pcri-P5UPcUbi4-2
(Plasmid
#255377)
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PurposePromoter + 5'UTR from Petroselinum crispum Polyubiquitin for tunable gene expression in monocots
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMTK
- Backbone size w/o insert (bp) 2794
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP5UPcUbi4-2
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SpeciesPetroselinum crispum
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Insert Size (bp)992
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMTK_026-Pcri-P5UPcUbi4-2 was a gift from Scott Lenaghan (Addgene plasmid # 255377 ; http://n2t.net/addgene:255377 ; RRID:Addgene_255377) -
For your References section:
Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility. May DB 3rd, Pfotenhauer AC, Concha BL, Li L, Jones SM, Harbison SA, Martin LH, Clark LA, Occhialini A, Stewart CN Jr, Lenaghan SC. Plant Cell Rep. 2025 Aug 8;44(9):196. doi: 10.1007/s00299-025-03583-x. 10.1007/s00299-025-03583-x PubMed 40779074