Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.
May DB 3rd, Pfotenhauer AC, Concha BL, Li L, Jones SM, Harbison SA, Martin LH, Clark LA, Occhialini A, Stewart CN Jr, Lenaghan SC
Plant Cell Rep. 2025 Aug 8;44(9):196. doi: 10.1007/s00299-025-03583-x.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255352 | pMTK_001-BRRV-PBR3 | Promoter fragment BR3 from Blueberry Red Ringspot Virus (BRRV) for tunable gene expression in monocots |
| 255353 | pMTK_002-BRRV-PBRRV | Promoter from Blueberry Red Ringspot Virus (BRRV) for tunable gene expression in monocots |
| 255354 | pMTK_003-BSV-PBSV | Promoter from Banana Streak Virus (BSV) for tunable gene expression in monocots |
| 255355 | pMTK_004-CERV-PCERV | Promoter from Carnation Etched Ringspot Virus (CERV) for tunable gene expression in monocots |
| 255356 | pMTK_005-ComYMV-P5UComYMV | Promoter + 5'UTR from Commelina Yellow Mottle Virus (ComYMV) for tunable gene expression in monocots |
| 255357 | pMTK_006-DaMV-PDaMV | Promoter from Dahlia Mosaic Virus (DaMV) for tunable gene expression in monocots |
| 255358 | pMTK_007-BRRV-PFBR3 | Synthetic promoter FBR3 derived from Blueberry Red Ringspot Virus (BRRV) for tunable gene expression in monocots |
| 255359 | pMTK_008-FMV-PFMV | Promoter from Figwort Mosaic Virus (FMV) for tunable gene expression in monocots |
| 255360 | pMTK_009-FMV34S-PFMV34S | Promoter from Figwort Mosaic Virus 34S (FMV34S) for tunable gene expression in monocots |
| 255361 | pMTK_010-MMV-PM24MMV | Promoter M24 from Mirabilis Mosaic Virus (MMV) for tunable gene expression in monocots |
| 255362 | pMTK_011-BRRV-PMBR3 | Synthetic promoter MBR3 derived from Blueberry Red Ringspot Virus (BRRV) for tunable gene expression in monocots |
| 255363 | pMTK_012-MMV-PMMV | Promoter from Mirabilis Mosaic Virus (MMV) for tunable gene expression in monocots |
| 255366 | pMTK_015-SbCMV-PSbCMV | Promoter from Soybean Chlorotic Mottle Virus (SbCMV) for tunable gene expression in monocots |
| 255367 | pMTK_016-SCBV-IM-PSCBV-IM | Promoter from Sugarcane Bacilliform Badnavirus Ireng Maleng Isolate (SCBV-IM) for tunable gene expression in monocots |
| 255368 | pMTK_017-SCBV-MOR-PSCBV-MOR | Promoter from Sugarcane Bacilliform Badnavirus Moroccan Isolate (SCBV-MOR) for tunable gene expression in monocots |
| 255369 | pMTK_018-SVBV-P5USVBV | Promoter from Strawberry Vein Banding Virus (SVBV) for tunable gene expression in monocots |
| 255370 | pMTK_019-Acom-P5UAcSUI1 | Promoter + 5'UTR from Ananas comosus translation factor SUI1 for tunable gene expression in monocots |
| 255372 | pMTK_021-Bdis-P5UBdUbi10 | Promoter + 5'UTR from Brachypodium distachyon Ubiquitin 10 for tunable gene expression in monocots |
| 255373 | pMTK_022-Clan-P5UClCula11 | Promoter + 5'UTR from Cunninghamia lanceolate for tunable gene expression in monocots |
| 255375 | pMTK_024-Osat-P5UOsCon1 | Promoter + 5'UTR from Oryza sativa cytosolic dehydroascorbate reductase cytDHAR for tunable gene expression in monocots |
| 255376 | pMTK_025-Osat-P5UOsUbi2 | Promoter + 5'UTR from Oryza sativa Ubiquitin 2 for tunable gene expression in monocots |
| 255377 | pMTK_026-Pcri-P5UPcUbi4-2 | Promoter + 5'UTR from Petroselinum crispum Polyubiquitin for tunable gene expression in monocots |
| 255379 | pMTK_028-Pvir-P5UPvUbi1+3 | Promoter + 5'UTR from Panicum virgatum Ubiquitin 1 + first 3 amino acids of CDS for tunable gene expression in monocots |
| 255381 | pMTK_030-TaBV-P5UTaBV T1200 | Promoter + 5'UTR from Taro Bacilliform Badnavirus (~1200 bp fragment) for tunable gene expression in monocots |
| 255382 | pMTK_031-TaBV-P5UTaBV T500 | Promoter + 5'UTR from Taro Bacilliform Badnavirus (~500 bp fragment) for tunable gene expression in monocots |
| 255383 | pMTK_032-TaBV-P5UTaBV T600 | Promoter + 5'UTR from Taro Bacilliform Badnavirus (~600 bp fragment) for tunable gene expression in monocots |
| 255384 | pMTK_033-Zmay-P5UZmUbi1 | Promoter + 5'UTR from Zea mays Ubiquitin 1 for tunable gene expression in monocots |
| 255385 | pMTK_034-BRRV-5BR3 | 5'UTR from Blueberry Red Ringspot Virus BR3 (BRRV) for tunable gene expression in monocots |
| 255386 | pMTK_035-FMV34S-5FMV34S | 5'UTR from Figwort Mosaic Virus 34S (FMV34S) for tunable gene expression in monocots |
| 255387 | pMTK_036-BRRV-5BRRV | 5'UTR from Blueberry Red Ringspot Virus (BRRV) for tunable gene expression in monocots |
| 255388 | pMTK_037-CERV-5CERV | 5'UTR from Carnation Etched Ringspot Virus (CERV) for tunable gene expression in monocots |
| 255389 | pMTK_038-DaMV-5DaMV | 5'UTR from Dahlia Mosaic Virus (DaMV) for tunable gene expression in monocots |
| 255390 | pMTK_039-FMV-5FMV | 5'UTR from Figwort Mosaic Virus (FMV) for tunable gene expression in monocots |
| 255391 | pMTK_040-BSV-5BSV | 5'UTR from Banana Streak Virus (BSV) for tunable gene expression in monocots |
| 255392 | pMTK_041-MMV-5MMV | 5'UTR from Mirabilis Mosaic Virus (MMV) for tunable gene expression in monocots |
| 255393 | pMTK_042-PClSV-5PClSV | 5'UTR from Peanut Chlorotic Streak Virus (PClSV) for tunable gene expression in monocots |
| 255394 | pMTK_043-PVCV-5PVCV | 5' UTR from Petunia Vein Clearing Virus (PVCV) for tunable gene expression in monocots |
| 255395 | pMTK_044-RTBV-5RTBV | 5'UTR from Rice Tungro Bacilliform Virus (RTBV) for tunable gene expression in monocots |
| 255396 | pMTK_045-SbCMV-5SbCMV | 5'UTR from Soybean Chlorotic Mottle Virus (SbCMV) for tunable gene expression in monocots |
| 255398 | pMTK_047-BYDV-5BYDV | 5UTR from Barley Yellow Dwarf Virus (BYDV) for tunable gene expression in monocots |
| 255399 | pMTK_048-TNVD-5TNVD | 5UTR from Tobacco Necrosis Virus-D (TNVD) for tunable gene expression in monocots |
| 255400 | pMTK_049-PEMV-5PEMV | 5UTR from Pea Enation Mosaic Virus (PEMV) for tunable gene expression in monocots |
| 255401 | pMTK_050-AlMV-5AlMV | 5UTR from Alfalfa Mosaic Virus RNA4 (AlMV) for tunable gene expression in monocots |
| 255402 | pMTK_051-Atha-3AtGRP | 3'UTR from Arabidopsis thaliana Glycine-rich protein for tunable gene expression in monocots |
| 255403 | pMTK_052-Atha-3AtHSP17.4 | 3'UTR from Arabidopsis thaliana Heat shock 17.4kDa protein for tunable gene expression in monocots |
| 255404 | pMTK_053-Atha-3AtHSP18.2 | 3'UTR from Arabidopsis thaliana Heat shock 18.2kDa protein for tunable gene expression in monocots |
| 255405 | pMTK_054-Atha-3AtPLAT1 | 3'UTR from Arabidopsis thaliana PLAT domain protein 1 for tunable gene expression in monocots |
| 255406 | pMTK_055-Bdis-3BdHSP16.9 | 3'UTR from Brachypodium distachyon Heat shock 16.9kDa protein for tunable gene expression in monocots |
| 255407 | pMTK_056-Bdis-3BdHSP17.2 | 3'UTR from Brachypodium distachyon Heat shock 17.2kDa protein for tunable gene expression in monocots |
| 255408 | pMTK_057-Bdis-3BdHSP70 | 3'UTR from Brachypodium distachyon Heat shock 70kDa protein for tunable gene expression in monocots |
| 255409 | pMTK_058-Psat-3PsRbcS3A | 3'UTR from Pisum sativum Rbcs3A (pea3A) for tunable gene expression in monocots |
| 255410 | pMTK_059-Pvir-3PvHSP17.5 | 3'UTR from Panicum virgatum Heat shock 17.5kDa protein for tunable gene expression in monocots |
| 255411 | pMTK_060-Pvir-3PvHSP70 | 3'UTR from Panicum virgatum Heat shock 70kDa protein for tunable gene expression in monocots |
| 255412 | pMTK_061-Sbic-3SbHSP16.9 | 3'UTR from Sorghum bicolor Heat shock 16.9kDa protein for tunable gene expression in monocots |
| 255413 | pMTK_062-Sbic-3SbHSP70 | 3'UTR from Sorghum bicolor Heat shock 70kDa protein for tunable gene expression in monocots |
| 255414 | pMTK_063-Soff-3ShHSP17.2 | 3'UTR from Saccharum officinarum x spontaneum Heat shock 17.2kDa protein for tunable gene expression in monocots |
| 255415 | pMTK_064-Soff-3ShHSP17.9 | 3'UTR from Saccharum officinarum x spontaneum Heat shock 17.9kDa protein for tunable gene expression in monocots |
| 255416 | pMTK_065-Soff-3ShHSP70 | 3'UTR from Saccharum officinarum x spontaneum Heat shock 70kDa protein for tunable gene expression in monocots |
| 255418 | pMTK_067-Svir-3SvHSP70 | 3'UTR from Setaria viridis Heat shock 70kDa protein for tunable gene expression in monocots |
| 255419 | pMTK_068-Zmay-3ZmG6PDH | 3'UTR from Zea mays Glucose-6-phosphate dehydrogenase 5 for tunable gene expression in monocots |
| 255420 | pMTK_069-Zmay-3ZmHSP70 | 3'UTR from Zea mays Heat shock 70kDa protein for tunable gene expression in monocots |