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Sp6-rpEca
(Plasmid #255429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255429 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV-Sport 6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4396
  • Total vector size (bp) 6343
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ProEcarin
  • Alt name
    Pro-Eca
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1848
  • GenBank ID
    D32212.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • Strep-tag (C terminal on insert)
    • Strep-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site AsiSI (not destroyed)
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sp6-rpEca was a gift from F Xavier Gomis-Ruth (Addgene plasmid # 255429 ; http://n2t.net/addgene:255429 ; RRID:Addgene_255429)

  • For your References section:

    A human-cell recombinant prethrombin-2 platform producing thrombin with enhanced fibrinogen processing for in vitro applications. Swaid H, Mendes SR, Fuentes-Prior P, Rodriguez-Banqueri A, Gomis-Ruth FX. Thromb Res. 2026 Oct 5;266:109863. doi: 10.1016/j.thromres.2026.109863. 10.1016/j.thromres.2026.109863 PubMed 42837787
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