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pCMV-HA-KDM6B-U
(Plasmid #255434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255434 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDM6B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6340
  • Entrez Gene
    KDM6B (a.k.a. JMJD3, NEDCFSA, NEDSST)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-KDM6B-U was a gift from Christine Mayr (Addgene plasmid # 255434 ; http://n2t.net/addgene:255434 ; RRID:Addgene_255434)

  • For your References section:

    mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity. Luo Y, Zhong Y, Basu S, Wu MC, Mayr C. Cell. 2026 Jun 8:S0092-8674(26)00576-3. doi: 10.1016/j.cell.2026.05.017. 10.1016/j.cell.2026.05.017 PubMed 42259285
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