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PSPPH0865
(Plasmid #255511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255511 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET 15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2-deoxyribose-5-phosphate aldolase (DERA) from Pseudomonas savastanoi pv. Phaseolicola
  • Alt name
    PSPPH0865
  • Alt name
    Q48N75
  • Species
    Pseudomonas savastanoi pv. Phaseolicola
  • Mutation
    WT
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag-TEV protease recognition site (ENLYFQ/G) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSPPH0865 was a gift from Radhakrishnan Mahadevan (Addgene plasmid # 255511 ; http://n2t.net/addgene:255511 ; RRID:Addgene_255511)

  • For your References section:

    Rational engineering of 2-deoxyribose-5-phosphate aldolases for the biosynthesis of (R)-1,3-butanediol. Kim T, Stogios PJ, Khusnutdinova AN, Nemr K, Skarina T, Flick R, Joo JC, Mahadevan R, Savchenko A, Yakunin AF. J Biol Chem. 2020 Jan 10;295(2):597-609. doi: 10.1074/jbc.RA119.011363. Epub 2019 Dec 5. 10.1074/jbc.RA119.011363 PubMed 31806708
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