Rational engineering of 2-deoxyribose-5-phosphate aldolases for the biosynthesis of (R)-1,3-butanediol.
Kim T, Stogios PJ, Khusnutdinova AN, Nemr K, Skarina T, Flick R, Joo JC, Mahadevan R, Savchenko A, Yakunin AF
J Biol Chem. 2020 Jan 10;295(2):597-609. doi: 10.1074/jbc.RA119.011363. Epub 2019 Dec 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255501 | BCE_1975 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255502 | BH1352 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255503 | BSU39420 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255504 | CV_3701 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255506 | LMO1995 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255507 | LPG1433 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255508 | LVIS_1595 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255509 | MTH_818 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255510 | PAE1231 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255512 | SAV2137 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255513 | SF4413 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255514 | SM11_pD0790 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255516 | SP_0843 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255517 | STM4567 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255518 | TM_1559 | DERA enzyme production under control of T7 promoter and inducible lac operator |
| 255519 | TTHA1186 | DERA enzyme production under control of T7 promoter and inducible lac operator |