Skip to main content
Addgene
Open Menu

pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald
(Plasmid #255634)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255634 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO-EYFP
  • Backbone manufacturer
    Karl Deisseroth (Addgene # 27056)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cx34.7
  • Species
    Morone americana (white perch fish)
  • Mutation
    E214K,E223K
  • Promoter EF1a
  • Tag / Fusion Protein
    • mEmerald (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald was a gift from Kafui Dzirasa (Addgene plasmid # 255634 ; http://n2t.net/addgene:255634 ; RRID:Addgene_255634)

  • For your References section:

    Long-term editing of brain circuits using an engineered electrical synapse. Ransey E, Thomas GE, Wisdom EM, Almoril-Porras A, Bowman R, Adamson E, Walder-Christensen KK, White JA, Hughes DN, Schwennesen H, Ferguson C, Tye KM, Mague SD, Niu L, Wang ZW, Colon-Ramos D, Hultman R, Bursac N, Dzirasa K. Nature. 2026 May 13. doi: 10.1038/s41586-026-10501-y. 10.1038/s41586-026-10501-y PubMed 42129559
Related items: