Long-term editing of brain circuits using an engineered electrical synapse.
Ransey E, Thomas GE, Wisdom EM, Almoril-Porras A, Bowman R, Adamson E, Walder-Christensen KK, White JA, Hughes DN, Schwennesen H, Ferguson C, Tye KM, Mague SD, Niu L, Wang ZW, Colon-Ramos D, Hultman R, Bursac N, Dzirasa K
Nature. 2026 May 13. doi: 10.1038/s41586-026-10501-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255632 | pAAV-CaMKII-Cx34.7-M1-mEmerald | Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by CaMKII promoter. |
| 255634 | pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald | Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by EF1a promoter. |
| 255638 | pAAV-hSyn-DIO-Cx35-M1-FLAG-T2A-mCherry | Encodes floxed (DIO) M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. hSyn promoter. |
| 255639 | pAAV-CaMKII-Cx35-M1-FLAG-T2A-mCherry | Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by CaMKII promoter. |
| 255640 | pAAV-hSyn-Cx35-M1-FLAG-T2A-mCherry | Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by hSyn promoter. |