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pAAV-hSyn-DIO-Cx35-M1-FLAG-T2A-mCherry
(Plasmid #255638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255638 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV-hSyn-DIO-EGFP
  • Backbone manufacturer
    Bryan Roth (Addgene # 50457)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cx35
  • Species
    Morone americana (white perch fish)
  • Mutation
    K221E
  • Promoter hSyn
  • Tag / Fusion Protein
    • FLAG-T2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-DIO-Cx35-M1-FLAG-T2A-mCherry was a gift from Kafui Dzirasa (Addgene plasmid # 255638 ; http://n2t.net/addgene:255638 ; RRID:Addgene_255638)

  • For your References section:

    Long-term editing of brain circuits using an engineered electrical synapse. Ransey E, Thomas GE, Wisdom EM, Almoril-Porras A, Bowman R, Adamson E, Walder-Christensen KK, White JA, Hughes DN, Schwennesen H, Ferguson C, Tye KM, Mague SD, Niu L, Wang ZW, Colon-Ramos D, Hultman R, Bursac N, Dzirasa K. Nature. 2026 May 13. doi: 10.1038/s41586-026-10501-y. 10.1038/s41586-026-10501-y PubMed 42129559
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