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pJT002 AAVS1-puroR-[RECloneSite]-minCMV-IGKleader-Cas9site-hIgG1_FC-MyC-PDGFRb-T2A-Citrine-SV40_PolyA
(Plasmid #255646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255646 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCL056
  • Backbone size (bp) 7229
  • Vector type
    Mammalian Expression
  • Promoter minCMV (minimal CMV promoter)
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Propagate in NEB Stable cells at 37°C. Avoid overgrowth to reduce recombination of repetitive elements.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oJT_pJT001_sanger_F
  • 3′ sequencing primer oJT_pJT001_sanger_R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from pCL056 (gift from L. Bintu Lab, Stanford University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT002 AAVS1-puroR-[RECloneSite]-minCMV-IGKleader-Cas9site-hIgG1_FC-MyC-PDGFRb-T2A-Citrine-SV40_PolyA was a gift from Jesse Engreitz (Addgene plasmid # 255646 ; http://n2t.net/addgene:255646 ; RRID:Addgene_255646)
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