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pCAG-RFP
(Plasmid #255759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255759 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEMT-Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3008
  • Total vector size (bp) 6042
  • Modifications to backbone
    No modifications were made to backbone. Insert was merely cloned into multiple cloning site of pGEMT-Easy.
  • Vector type
    Mammalian Expression ; Reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CAG promoter expressing human H2B linked to mRFP1 reporter protein
  • Insert Size (bp)
    3034
  • Mutation
    N/A
  • Tag / Fusion Protein
    • mRFP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer M13 fwd: 5'-GTAAAACGACGGCCAGT-3'
  • 3′ sequencing primer M13 rev: 5'-CAGGAAACAGCTATGAC-3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-RFP was a gift from Natalya Nikitina (Addgene plasmid # 255759 ; http://n2t.net/addgene:255759 ; RRID:Addgene_255759)

  • For your References section:

    Chick gonad explant electroporation reveals enhancers for testes determining factor, DMRT1. Oberholzer Z, Taylor G, Hockman D, Nikitina NV. Dev Biol. 2026 Jun;534:160-172. doi: 10.1016/j.ydbio.2026.03.011. Epub 2026 Mar 17. 10.1016/j.ydbio.2026.03.011 PubMed 41850651
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