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Chick gonad explant electroporation reveals enhancers for testes determining factor, DMRT1.

Oberholzer Z, Taylor G, Hockman D, Nikitina NV
Dev Biol. 2026 Jun;534:160-172. doi: 10.1016/j.ydbio.2026.03.011. Epub 2026 Mar 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
255759pCAG-RFPGeneral tracer plasmid. RFP expressed under the ubiquitous CAG promoter
255760Tol2-pTK-HS4x4Tol2-transposon based enhancer reporter construct with insulators. Putative enhancers are inserted upstream the minimal promoter which is linked to GFP reporter. Active enhancers drive GFP expression.
255761Tol2-TKDual colour, Tol2-transposon based enhancer reporter construct capable of long term screening. RFP acts as a tracer and GFP indicates active enhancers. Insulators prevent epigenetic silencing.

Antibodies from Article