Tol2-TK
(Plasmid #255761)

• Purpose: Dual colour, Tol2-transposon based enhancer reporter construct capable of long term screening. RFP acts as a tracer and GFP indicates active enhancers. Insulators prevent epigenetic silencing.
• Depositing Lab: Natalya Nikitina
• Publication: Oberholzer et al Dev Biol. 2026 Jun;534:160-172. doi: 10.1016/j.ydbio.2026.03.011. Epub 2026 Mar 17.

Backbone

• Vector backbone: pHLC
• Backbone size w/o insert (bp): 5376
• Total vector size (bp): 9941
• Vector type: Mammalian Expression ; Enhancer reporter construct

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: HSV TK minimal promoter linked to GFP reporter protein
• Insert Size (bp): 1527
• Tag / Fusion Protein:
  • GFP

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: CAG promoter expressing human H2B linked to mRFP1 reporter protein
• Insert Size (bp): 3037
• Tag / Fusion Protein:
  • mRFP1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry